Porcine Reproductive and Respiratory Syndrome Virus Ab (PRRSV Ab)
Test
Application:
The PRRSV Antibody Rapid Test Cassette is a rapid chromatographic
immunoassay for the qualitative detection of antibodies to Porcine
Reproductive and Respiratory Syndrome Virus in serum or plasma.
| Principle: | Chromatographic Immunoassay |
| Specimen: | S/P |
| Pack: | 10 T |
| Shelf Life: | 2 Years |
| Specificity: | 92.90% |
| Format: | Cassette |
| Reading Time: | 10 Minutes |
| Storage Temperature: | 4-30℃ |
| Sensitivity: | 97.50% |
| Accuracy: | 95.60% |
Description:
The PRRSV Antibody Rapid Test Cassette (Serum/Plasma) is a
qualitative, membrane based immunoassay with a double antigen
system for the detection of antibodies to Porcine Reproductive &
Respiratory Syndrome Virus in serum or plasma. The membrane is
pre-coated with recombinant PRRSV antigens. During testing,
antibodies to PRRSV, if present in serum or plasma specimen react
with pre-coated PRRSV antigen in the test strip.
The mixture then migrates upward on the membrane
chromatographically by capillary action and reacts with recombinant
PRRSV antigens on the membrane in the test line region. If the
specimen contains antibodies to Porcine Reproductive & Respiratory
Virus, a colored line will appear in the test line region
indicating a positive result.
If the specimen does not contain Porcine Reproductive & Respiratory
Virus antibodies, a colored line will not appear in the test line
region indicating a negative result. To serve as a procedural
control, a colored line will always appear in the control line
region indicating that proper volume of specimen has been added and
membrane wicking has occurred.
How to use?
- Remove the test cassette from the foil pouch and use it as soon as
possible. Best results will be obtained if the assay is performed
within one hour.
- Collect fresh whole blood, and separate serum or plasma from blood
as soon as possible to avoid hemolysis. Only use clear,
non-hemolyzed specimens. Do not leave the specimens at room
temperature for prolonged periods. Serum and plasma specimens may
be stored at 2-8 °C for up to 3 days. For long term storage,
specimens should be kept below -20 °C.
- Place the test cassette on a clean and level surface. Hold the
dropper vertically and transfer 3 drops of serum or plasma (approximately 75 μl) to the specimen well (S) of the test
cassette, then start the timer. See illustration below.
- Read the results at 10 minutes. Do not interpret results after 20 minutes.
Order Information:
| Cat. No. | Product | Specimen | Pack |
| VIPRR-302 | Porcine Reproductive and Respiratory Syndrome Virus Antibody (PRRSV
Ab) Test | S / P | 10T |
INTERPRETATION OF RESULTS
Positive: The presence of both C line and T line, regardless of T
line being strong or faint.
Negative: Only clear C line appears.
Invalid: No colored line appears in C line region, regardless of T
line’s appearance.
